The Frank L. Conlon Lab
Department of Biological & Genome Sciences
McAllister Heart Institute
University of North Carolina at Chapel Hill
School of Medicine & College of Arts and Sciences
Publications
Edwards, W., et al. (2023) Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development. Developmental Cell 58: 1-19. PMID: 37148880
Edwards, W., et al. (2023) The TBX20-TLE interaction is essential for the second heart field. Development. 2023 Nov 1;150(21):dev201677.
Emerson, J., et al, (2023) Sex Differences in Mouse Cardiac Electrophysiology Revealed by Simultaneous Imaging of Excitation-Contraction Coupling. Journal of Cardiac Development and Disease. In Press.
Shi, W., et al, (2024) CHD4 and SMYD1 repress common transcriptional programs in the developing heart. Development. In Press
Conlon, F.L. and Arnold, A.P. (2023) Sex chromosome mechanisms in cardiac development and disease. Nature Cardiovascular Research 2, 340-350. PMID: 37808586
Shi, W., et al. (2023) A missense mutation in human CHD4 causes ventricular noncompaction by repressing ADAMTS1-mediated trabeculation cessation. Circulation Research 133: 48-67. PMID: 37254794
Robbe, Z., Shi, W., et al. (2022) CHD4 is recruited by GATA4 and NKX2-5 to repress non-cardiac gene programs in the developing heart. Genes & Development 36: 468-482. PMID: 35450884.
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Shi, W., et al. (2021) Cardiac proteomics reveals sex chromosome-dependent differences between males and
females that arise prior to gonad formation. Developmental Cell S1534-5807(21)00766-8. PMID: 34655525.
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​Sigmon, JS, et al. (2020) Content and Performance of the MiniMUGA Genotyping Array: A New Tool to Improve Rigor and Reproducibility in Mouse Research. Genetics 216(4): 905-930. PMID: 33067325
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Conlon, Fl. L., Slagle, C. E., Wessels, A. (2019). Basic Anatomy and Embryology of the Heart. Netter's Cardiology, 3rd edition. edn. Stouffer, Runge, Patterson, Rossi (Philadelphia, PA: Elsevier).
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Akerberg BN, et al. (2019) Cardiac transcription factor chromatin occupancy map: a reference map of cardiac transcription factor chromatin occupancy identifies dynamic and conserved transcriptional enhancers. Nature Communications. Oct 28: 10(1):4907. PMID: 31659164
Hoppler, S and Conlon, F. L. (2019) Experimental Access to Cardiovascular Development, Regeneration Discovery, and Cardiovascular Heart-Defect Modeling. In Heart Disease and Development. Cold Spring Harbor Perspectives, Eds. Riley and Bruneau. Nov 25: a037200. PMID: 31767648
Federspiel, J. D., Tandon, P., Wilczewski, C. W., et al., (2019). Conservation and divergence of protein pathways in the vertebrate heart. PLoS Biology. Sep 6;17(9:e3000437). PMID: 31490923
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Dorr, K. M. and Conlon, F. L. (2019) Proteomic-Based Approaches to Cardiac Development and Disease. Current Opinion in Chemical Bio. Feb;48:150-157. PMID: 30711722
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Steimle et al., (2019) An evolutionarily conserved Tbx5-Wnt2/2b pathway orchestrates cardiopulmonary development. Proc Natl Acad Sci USA 115 (45). PMID: 30352852.
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Wasson L, Amin NM, & Conlon FL (2019) INTACT Proteomics in Xenopus. Cold Spring Harb Protoc 2019 Jun 3;2019(6): pdb.prot098384. PMID: 30150318
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Wilczewski CM, et al. (2018) CHD4 and the NuRD complex directly control cardiac sarcomere formation. Proc Natl Acad Sci USA 115(26):6727-6732. PMID: 29891665
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Sauls K, et al. (2018) Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Reports 22(7):1913-1922. PMID: 29444441
Lema Asqui S, et al. (2018) AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta. New Phytologist 218(3):1156-1166. PMID: 28157265
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Tandon P, Conlon F, Furlow JD, & Horb ME (2017) Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling. Developmental Cell Biology 426(2):325-335. PMID: 27109192
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Kennedy L, et al. (2017) Formation of a TBX20-CASZ1 protein complex is protective against dilated cardiomyopathy and critical for cardiac homeostasis. PLoS Genetics 13(9):e1007011. PMID: 28945738
Waldron L, et al. (2016) The Cardiac TBX5 Interactome Reveals a Chromatin Remodeling Network Essential for Cardiac Septation. Developmental Cell 36(3):262-275. PMID: 26859351
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Tandon P, Wilczewski CM, Williams CE, & Conlon FL (2016) The Lhx9-integrin pathway is essential for positioning of the proepicardial organ. Development 143(5):831-840. PMID: 26811386
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Sullivan-Brown JL, et al. (2016) Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation. Genetics 202(1):123-139. PMID: 26434722
Slagle CE & Conlon FL (2016) Emerging Field of Cardiomics: High-Throughput Investigations into Transcriptional Regulation of Cardiovascular Development and Disease. Trends in Genetetics 32(11):707-716. PMID: 27717505
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Dorr KM, et al. (2015) Casz1 is required for cardiomyocyte G1-to-S phase progression during mammalian cardiac development. Development 142(11):2037-2047. PMID: 25953344
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Charpentier MS, Tandon P, Trincot CE, Koutleva EK, & Conlon FL (2015) A distinct mechanism of vascular lumen formation in Xenopus requires EGFL7. PLoS One 10(2):e0116086. PMID: 25705891
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Sojka S, et al. (2014) Congenital heart disease protein 5 associates with CASZ1 to maintain myocardial tissue integrity. Development 141(15):3040-3049. PMID: 24993940
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Charpentier MS & Conlon FL (2014) Cellular and molecular mechanisms underlying blood vessel lumen formation. Bioessays 36(3):251-259. PMID: 24323945
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Amin NM, Tandon P, Osborne Nishimura E, & Conlon FL (2014) RNA-seq in the tetraploid Xenopus laevis enables genome-wide insight in a classic developmental biology model organism. Methods 66(3):398-409. PMID: 23792920
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Amin NM, et al. (2014) Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT). Development 141(4):962-973. PMID: 24496632
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Amin NM, Gibbs D, & Conlon FL (2014) Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development. Developmental Dynamics 243(7):948-956. PMID: 24633745
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Tandon P, Miteva YV, Kuchenbrod LM, Cristea IM, & Conlon FL (2013) Tcf21 regulates the specification and maturation of proepicardial cells. Development 140(11):2409-2421. PMID: 23637334
Kaltenbrun E, et al. (2013) A Gro/TLE-NuRD corepressor complex facilitates Tbx20-dependent transcriptional repression. J Proteome Res 12(12):5395-5409. PMID: 24024827
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Charpentier MS, Taylor JM, & Conlon FL (2013) The CASZ1/Egfl7 transcriptional pathway is required for RhoA expression in vascular endothelial cells. Small GTPases 4(4):231-235. PMID: 24150064
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Charpentier MS, Dorr KM, & Conlon FL (2013) Transcriptional regulation of blood vessel formation: the role of the CASZ1/Egfl7/RhoA pathway. Cell Cycle 12(14):2165-2166. PMID: 23803731
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Charpentier MS, et al. (2013) CASZ1 promotes vascular assembly and morphogenesis through the direct regulation of an EGFL7/RhoA-mediated pathway. Developmental Cell 25(2):132-143. PMID: 23639441
Tandon P, Showell C, Christine K, & Conlon FL (2012) Morpholino injection in Xenopus. Methods Molecular Biol 843:29-46. PMID: 22222519
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Tandon P, Conlon FL, & Taylor JM (2012) ROCKs cause SHP-wrecks and broken hearts. Small GTPases 3(4):209-212. PMID: 22858643
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Langdon Y, et al. (2012) SHP-2 acts via ROCK to regulate the cardiac actin cytoskeleton. Development 139(5):948-957. PMID: 22278918
Greco TM, Miteva Y, Conlon FL, & Cristea IM (2012) Complementary proteomic
analysis of protein complexes. Methods Molecular Biol 917:391-407. PMID: 22956100
Conlon FL, et al. (2012) Immunoisolation of protein complexes from Xenopus. Methods Molecular Biol 917:369-390. PMID: 22956099
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Showell C, et al. (2011) A comparative survey of the frequency and distribution of polymorphism in the genome of Xenopus tropicalis. PLoS One 6(8):e22392. PMID: 21829622
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Kaltenbrun E, et al. (2011) Xenopus: An emerging model for studying congenital heart disease. Birth Defects Res A Clin Mol Teratol 91(6):495-510. PMID: 21538812
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Guo Y, Christine KS, Conlon F, Gessert S, & Kuhl M (2011) Expression analysis of epb41l4a during Xenopus laevis embryogenesis. Development Genes & Evolution 221(2):113-119. PMID: 21556855
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Gee ST, Milgram SL, Kramer KL, Conlon FL, & Moody SA (2011) Yes-associated protein 65 (YAP) expands neural progenitors and regulates Pax3 expression in the neural plate border zone. PLoS One 6(6):e20309. PMID: 21687713
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Doherty JT, et al. (2011) Skeletal muscle differentiation and fusion are regulated by the BAR-containing Rho-GTPase-activating protein (Rho-GAP), GRAF1. J Biol Chem 286(29):25903-25921. PMID: 21622574
Mandel EM, et al. (2010) The BMP pathway acts to directly regulate Tbx20 in the developing heart. Development 137(11):1919-1929. PMID: 20460370
Evans SM, Yelon D, Conlon FL, & Kirby ML (2010) Myocardial lineage development. Circulation Research 107(12):1428-1444. PMID: 21148449
Doherty JT, Conlon FL, Mack CP, & Taylor JM (2010) Focal adhesion kinase is essential for cardiac looping and multichamber heart formation. Genesis 48(8):492-504. PMID: 20572259
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Conlon FL & Yutzey KE (2010) T-Box Factors. Heart Development and Regeneration, eds Rosenthal N & Harvey RP (Elsevier Inc.), pp 651-671. ScienceDirect link
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Showell C & Conlon FL (2009) The western clawed frog (Xenopus tropicalis): an emerging vertebrate model for developmental genetics and environmental toxicology. Cold Spring Harb Protoc 2009(9):pdb emo131. PMID: 20147259
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Showell C & Conlon FL (2009) Natural mating and tadpole husbandry in the western clawed frog Xenopus tropicalis. Cold Spring Harb Protoc 2009(9):pdb prot5292. PMID: 20147277
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Showell C & Conlon FL (2009) Egg collection and in vitro fertilization of the western clawed frog Xenopus tropicalis. Cold Spring Harb Protoc 2009(9):pdb prot5293. PMID: 20147278
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Showell C & Conlon FL (2009) Tissue sampling and genomic DNA purification from the western clawed frog Xenopus tropicalis. Cold Spring Harb Protoc 2009(9):pdb prot5294. PMID: 20147279
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Christine KS & Conlon FL (2008) Vertebrate CASTOR is required for differentiation of cardiac precursor cells at the ventral midline. Developmental Cell 14(4):616-623. PMID: 18410736
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Showell C & Conlon FL (2007) Decoding development in Xenopus tropicalis. Genesis 45(6):418-426. PMID: 17549727
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Langdon YG, Goetz SC, Berg AE, Swanik JT, & Conlon FL (2007) SHP-2 is required for the maintenance of cardiac progenitors. Development 134(22):4119-4130. PMID: 17928416
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Goetz SC & Conlon FL (2007) Cardiac progenitors and the embryonic cell cycle. Cell Cycle 6(16):1974-1981. PMID: 17712230
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Brown DD, Christine KS, Showell C, & Conlon FL (2007) Small heat shock protein Hsp27 is required for proper heart tube formation. Genesis 45(11):667-678. PMID: 17987658
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Showell C, Christine KS, Mandel EM, & Conlon FL (2006) Developmental expression patterns of Tbx1, Tbx2, Tbx5, and Tbx20 in Xenopus tropicalis. Developmental Cell Dynamics 235(6):1623-1630. PMID: 16477648
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Goetz SC, Brown DD, & Conlon FL (2006) TBX5 is required for embryonic cardiac cell cycle progression. Development 133(13):2575-2584. PMID: 16728474
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Chen JF, et al. (2006) The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nature Genetics 38(2):228-233. PMID:16380711
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Brown DD, Davis AC, & Conlon FL (2006) Xtn3 is a developmentally expressed cardiac and skeletal muscle-specific novex-3 titin isoform. Gene Expr Patterns 6(8):913-918. PMID: 16630749
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Mandel EM, Callis TY, Wang DZ, & Conlon FL (2005) Transcriptional mechanisms of congenital heart disease. Drug Discov Today 2(1):33-38. ScienceDirect link
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Brown DD, et al. (2005) Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis. Development 132(3):553-563. PMID: 15634698
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Showell C, Binder O, & Conlon FL (2004) T-box genes in early embryogenesis. Developmental Cell Dynamics 229(1):201-218. PMID: 14699590
Moser M, et al. (2003) BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation. Mol Cell Biol 23(16):5664-5679. PMID: 12897139
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Brown DD, Binder O, Pagratis M, Parr BA, & Conlon FL (2003) Developmental expression of the Xenopus laevis Tbx20 orthologue. Developmental Genes Evol 212(12):604-607. PMID: 12536325
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Wilson V & Conlon FL (2002) The T-box family. Genome Biol 3(6):REVIEWS3008.1-7. PMID: 12093383
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Conlon FL, Fairclough L, Price BM, Casey ES, & Smith JC (2001) Determinants of T box protein specificity. Development 128(19):3749-3758. PMID: 11585801
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Smith JC, Conlon FL, Saka Y, & Tada M (2000) Xwnt11 and the regulation of gastrulation in Xenopus. Philos Trans R Soc Lond B Biol Sci 355(1399):923-930. PMID: 11128985
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Conlon FL & Kessler DS (2000) Hopping into the new millennium. Eighth International Xenopus Conference, Estes Park, Colorado, 16-20 August 2000. Trends Genet 16(12):537-540. PMID: 11188367
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Conlon FL & Smith JC (1999) Interference with brachyury function inhibits convergent extension, causes apoptosis, and reveals separate requirements in the FGF and activin signalling pathways. Developmental Cell Biol 213(1):85-100. PMID: 10452848
Casey ES, O'Reilly MA, Conlon FL, & Smith JC (1998) The T-box transcription factor Brachyury regulates expression of eFGF through binding to a non-palindromic response element. Development 125(19):3887-3894. PMID: 9729496
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Smith JC, et al. (1997) Upstream and downstream from Brachyury, a gene required for vertebrate mesoderm formation. Cold Spring Harb Symp QuantBiol 62:337-346. PMID: 9598367
Morgan BA, et al. (1996) Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse. Proc Natl Acad Sci U S A 93(7):2801-2806. PMID: 8610121
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Conlon FL, Sedgwick SG, Weston KM, & Smith JC (1996) Inhibition of Xbra transcription activation causes defects in mesodermal patterning and reveals autoregulation of Xbra in dorsal mesoderm. Development 122(8):2427-2435. PMID: 8756288
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Conlon FL, Jones CM, & Smith JC (1996) From mouse to frogs: identification and functional analyses of genes required for induction and patterning of the mesoderm. Semin Developmental Cell Biology 7(1):95-101. ScienceDirect link
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Conlon FL, Wright CV, & Robertson EJ (1995) Effects of the TWis mutation on notochord formation and mesodermal patterning. Mech Dev 49(3):201-209. PMID: 7734393
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Conlon FL & Beddington RS (1995) Mouse gastrulation from a frog's perspective. Semin Developmental Cell Biol 6(4):249-256. ScienceDirect link
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Ruiz JC, Conlon FL, & Robertson EJ (1994) Identification of novel protein kinases expressed in the myocardium of the developing mouse heart. Mech Dev 48(3):153-164. PMID: 7893599
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Conlon FL, et al. (1994) A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. Development 120(7):1919-1928. PMID: 7924997
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Robertson EJ, Conlon FL, Barth KS, Costantini F, & Lee JJ (1992) Use of embryonic stem cells to study mutations affecting postimplantation development in the mouse. Ciba Found Symp 165:237-250; discussion 250-5. PMID: 1516471
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Conlon FL, Barth KS, & Robertson EJ (1991) A novel retrovirally induced embryonic lethal mutation in the mouse: assessment of the developmental fate of embryonic stem cells homozygous for the 413.d proviral integration. Development 111(4):969-981. PMID: 1879365