The Frank L. Conlon Lab
Department of Biological & Genome Sciences
McAllister Heart Institute
University of North Carolina at Chapel Hill
School of Medicine & College of Arts and Sciences
Publications
Edwards, W., et al. (2023) Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development. Developmental Cell 58: 1-19. PMID: 37148880
Edwards, W., et al. (2023) The TBX20-TLE interaction is essential for the second heart field. Development 150(21):dev201677. PMID: 37756602
Emerson, J., et al, (2023) Sex Differences in Mouse Cardiac Electrophysiology Revealed by Simultaneous Imaging of Excitation-Contraction Coupling. Journal of Cardiac Development and Disease 10(12):479. PMID: 38132647
Shi, W., et al, (2024) CHD4 and SMYD1 repress common transcriptional programs in the developing heart. Development. 151(8):dev202505. PMID: 38619323
Conlon, F.L. and Arnold, A.P. (2023) Sex chromosome mechanisms in cardiac development and disease. Nature Cardiovascular Research 2, 340-350. PMID: 37808586
Shi, W., et al. (2023) A missense mutation in human CHD4 causes ventricular noncompaction by repressing ADAMTS1-mediated trabeculation cessation. Circulation Research 133: 48-67. PMID: 37254794
Robbe, Z., Shi, W., et al. (2022) CHD4 is recruited by GATA4 and NKX2-5 to repress non-cardiac gene programs in the developing heart. Genes & Development 36: 468-482. PMID: 35450884.
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Shi, W., et al. (2021) Cardiac proteomics reveals sex chromosome-dependent differences between males and
females that arise prior to gonad formation. Developmental Cell S1534-5807(21)00766-8. PMID: 34655525.
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​Sigmon, JS, et al. (2020) Content and Performance of the MiniMUGA Genotyping Array: A New Tool to Improve Rigor and Reproducibility in Mouse Research. Genetics 216(4): 905-930. PMID: 33067325
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Conlon, Fl. L., Slagle, C. E., Wessels, A. (2019). Basic Anatomy and Embryology of the Heart. Netter's Cardiology, 3rd edition. edn. Stouffer, Runge, Patterson, Rossi (Philadelphia, PA: Elsevier).
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Akerberg BN, et al. (2019) Cardiac transcription factor chromatin occupancy map: a reference map of cardiac transcription factor chromatin occupancy identifies dynamic and conserved transcriptional enhancers. Nature Communications. Oct 28: 10(1):4907. PMID: 31659164
Hoppler, S and Conlon, F. L. (2019) Experimental Access to Cardiovascular Development, Regeneration Discovery, and Cardiovascular Heart-Defect Modeling. In Heart Disease and Development. Cold Spring Harbor Perspectives, Eds. Riley and Bruneau. Nov 25: a037200. PMID: 31767648
Federspiel, J. D., Tandon, P., Wilczewski, C. W., et al., (2019). Conservation and divergence of protein pathways in the vertebrate heart. PLoS Biology. Sep 6;17(9:e3000437). PMID: 31490923
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Dorr, K. M. and Conlon, F. L. (2019) Proteomic-Based Approaches to Cardiac Development and Disease. Current Opinion in Chemical Bio. Feb;48:150-157. PMID: 30711722
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Steimle et al., (2019) An evolutionarily conserved Tbx5-Wnt2/2b pathway orchestrates cardiopulmonary development. Proc Natl Acad Sci USA 115 (45). PMID: 30352852.
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Wasson L, Amin NM, & Conlon FL (2019) INTACT Proteomics in Xenopus. Cold Spring Harb Protoc 2019 Jun 3;2019(6): pdb.prot098384. PMID: 30150318
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Wilczewski CM, et al. (2018) CHD4 and the NuRD complex directly control cardiac sarcomere formation. Proc Natl Acad Sci USA 115(26):6727-6732. PMID: 29891665
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Sauls K, et al. (2018) Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Reports 22(7):1913-1922. PMID: 29444441
Lema Asqui S, et al. (2018) AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta. New Phytologist 218(3):1156-1166. PMID: 28157265
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Tandon P, Conlon F, Furlow JD, & Horb ME (2017) Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling. Developmental Cell Biology 426(2):325-335. PMID: 27109192
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Kennedy L, et al. (2017) Formation of a TBX20-CASZ1 protein complex is protective against dilated cardiomyopathy and critical for cardiac homeostasis. PLoS Genetics 13(9):e1007011. PMID: 28945738
Waldron L, et al. (2016) The Cardiac TBX5 Interactome Reveals a Chromatin Remodeling Network Essential for Cardiac Septation. Developmental Cell 36(3):262-275. PMID: 26859351
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Tandon P, Wilczewski CM, Williams CE, & Conlon FL (2016) The Lhx9-integrin pathway is essential for positioning of the proepicardial organ. Development 143(5):831-840. PMID: 26811386
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Sullivan-Brown JL, et al. (2016) Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation. Genetics 202(1):123-139. PMID: 26434722
Slagle CE & Conlon FL (2016) Emerging Field of Cardiomics: High-Throughput Investigations into Transcriptional Regulation of Cardiovascular Development and Disease. Trends in Genetetics 32(11):707-716. PMID: 27717505
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Dorr KM, et al. (2015) Casz1 is required for cardiomyocyte G1-to-S phase progression during mammalian cardiac development. Development 142(11):2037-2047. PMID: 25953344
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Charpentier MS, Tandon P, Trincot CE, Koutleva EK, & Conlon FL (2015) A distinct mechanism of vascular lumen formation in Xenopus requires EGFL7. PLoS One 10(2):e0116086. PMID: 25705891
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Sojka S, et al. (2014) Congenital heart disease protein 5 associates with CASZ1 to maintain myocardial tissue integrity. Development 141(15):3040-3049. PMID: 24993940
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Charpentier MS & Conlon FL (2014) Cellular and molecular mechanisms underlying blood vessel lumen formation. Bioessays 36(3):251-259. PMID: 24323945
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Amin NM, Tandon P, Osborne Nishimura E, & Conlon FL (2014) RNA-seq in the tetraploid Xenopus laevis enables genome-wide insight in a classic developmental biology model organism. Methods 66(3):398-409. PMID: 23792920
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Amin NM, et al. (2014) Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT). Development 141(4):962-973. PMID: 24496632
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Amin NM, Gibbs D, & Conlon FL (2014) Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development. Developmental Dynamics 243(7):948-956. PMID: 24633745
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Tandon P, Miteva YV, Kuchenbrod LM, Cristea IM, & Conlon FL (2013) Tcf21 regulates the specification and maturation of proepicardial cells. Development 140(11):2409-2421. PMID: 23637334
Kaltenbrun E, et al. (2013) A Gro/TLE-NuRD corepressor complex facilitates Tbx20-dependent transcriptional repression. J Proteome Res 12(12):5395-5409. PMID: 24024827
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Charpentier MS, Taylor JM, & Conlon FL (2013) The CASZ1/Egfl7 transcriptional pathway is required for RhoA expression in vascular endothelial cells. Small GTPases 4(4):231-235. PMID: 24150064
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Charpentier MS, Dorr KM, & Conlon FL (2013) Transcriptional regulation of blood vessel formation: the role of the CASZ1/Egfl7/RhoA pathway. Cell Cycle 12(14):2165-2166. PMID: 23803731
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Charpentier MS, et al. (2013) CASZ1 promotes vascular assembly and morphogenesis through the direct regulation of an EGFL7/RhoA-mediated pathway. Developmental Cell 25(2):132-143. PMID: 23639441
Tandon P, Showell C, Christine K, & Conlon FL (2012) Morpholino injection in Xenopus. Methods Molecular Biol 843:29-46. PMID: 22222519
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Tandon P, Conlon FL, & Taylor JM (2012) ROCKs cause SHP-wrecks and broken hearts. Small GTPases 3(4):209-212. PMID: 22858643
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Langdon Y, et al. (2012) SHP-2 acts via ROCK to regulate the cardiac actin cytoskeleton. Development 139(5):948-957. PMID: 22278918
Greco TM, Miteva Y, Conlon FL, & Cristea IM (2012) Complementary proteomic
analysis of protein complexes. Methods Molecular Biol 917:391-407. PMID: 22956100
Conlon FL, et al. (2012) Immunoisolation of protein complexes from Xenopus. Methods Molecular Biol 917:369-390. PMID: 22956099
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Showell C, et al. (2011) A comparative survey of the frequency and distribution of polymorphism in the genome of Xenopus tropicalis. PLoS One 6(8):e22392. PMID: 21829622
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Kaltenbrun E, et al. (2011) Xenopus: An emerging model for studying congenital heart disease. Birth Defects Res A Clin Mol Teratol 91(6):495-510. PMID: 21538812
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Guo Y, Christine KS, Conlon F, Gessert S, & Kuhl M (2011) Expression analysis of epb41l4a during Xenopus laevis embryogenesis. Development Genes & Evolution 221(2):113-119. PMID: 21556855
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Gee ST, Milgram SL, Kramer KL, Conlon FL, & Moody SA (2011) Yes-associated protein 65 (YAP) expands neural progenitors and regulates Pax3 expression in the neural plate border zone. PLoS One 6(6):e20309. PMID: 21687713
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Doherty JT, et al. (2011) Skeletal muscle differentiation and fusion are regulated by the BAR-containing Rho-GTPase-activating protein (Rho-GAP), GRAF1. J Biol Chem 286(29):25903-25921. PMID: 21622574
Mandel EM, et al. (2010) The BMP pathway acts to directly regulate Tbx20 in the developing heart. Development 137(11):1919-1929. PMID: 20460370
Evans SM, Yelon D, Conlon FL, & Kirby ML (2010) Myocardial lineage development. Circulation Research 107(12):1428-1444. PMID: 21148449
Doherty JT, Conlon FL, Mack CP, & Taylor JM (2010) Focal adhesion kinase is essential for cardiac looping and multichamber heart formation. Genesis 48(8):492-504. PMID: 20572259
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Conlon FL & Yutzey KE (2010) T-Box Factors. Heart Development and Regeneration, eds Rosenthal N & Harvey RP (Elsevier Inc.), pp 651-671. ScienceDirect link
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Showell C & Conlon FL (2009) The western clawed frog (Xenopus tropicalis): an emerging vertebrate model for developmental genetics and environmental toxicology. Cold Spring Harb Protoc 2009(9):pdb emo131. PMID: 20147259
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Showell C & Conlon FL (2009) Natural mating and tadpole husbandry in the western clawed frog Xenopus tropicalis. Cold Spring Harb Protoc 2009(9):pdb prot5292. PMID: 20147277
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Showell C & Conlon FL (2009) Egg collection and in vitro fertilization of the western clawed frog Xenopus tropicalis. Cold Spring Harb Protoc 2009(9):pdb prot5293. PMID: 20147278
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Showell C & Conlon FL (2009) Tissue sampling and genomic DNA purification from the western clawed frog Xenopus tropicalis. Cold Spring Harb Protoc 2009(9):pdb prot5294. PMID: 20147279
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Christine KS & Conlon FL (2008) Vertebrate CASTOR is required for differentiation of cardiac precursor cells at the ventral midline. Developmental Cell 14(4):616-623. PMID: 18410736
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Showell C & Conlon FL (2007) Decoding development in Xenopus tropicalis. Genesis 45(6):418-426. PMID: 17549727
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Langdon YG, Goetz SC, Berg AE, Swanik JT, & Conlon FL (2007) SHP-2 is required for the maintenance of cardiac progenitors. Development 134(22):4119-4130. PMID: 17928416
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Goetz SC & Conlon FL (2007) Cardiac progenitors and the embryonic cell cycle. Cell Cycle 6(16):1974-1981. PMID: 17712230
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Brown DD, Christine KS, Showell C, & Conlon FL (2007) Small heat shock protein Hsp27 is required for proper heart tube formation. Genesis 45(11):667-678. PMID: 17987658
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Showell C, Christine KS, Mandel EM, & Conlon FL (2006) Developmental expression patterns of Tbx1, Tbx2, Tbx5, and Tbx20 in Xenopus tropicalis. Developmental Cell Dynamics 235(6):1623-1630. PMID: 16477648
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Goetz SC, Brown DD, & Conlon FL (2006) TBX5 is required for embryonic cardiac cell cycle progression. Development 133(13):2575-2584. PMID: 16728474
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Chen JF, et al. (2006) The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nature Genetics 38(2):228-233. PMID:16380711
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Brown DD, Davis AC, & Conlon FL (2006) Xtn3 is a developmentally expressed cardiac and skeletal muscle-specific novex-3 titin isoform. Gene Expr Patterns 6(8):913-918. PMID: 16630749
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Mandel EM, Callis TY, Wang DZ, & Conlon FL (2005) Transcriptional mechanisms of congenital heart disease. Drug Discov Today 2(1):33-38. ScienceDirect link
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Brown DD, et al. (2005) Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis. Development 132(3):553-563. PMID: 15634698
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Showell C, Binder O, & Conlon FL (2004) T-box genes in early embryogenesis. Developmental Cell Dynamics 229(1):201-218. PMID: 14699590
Moser M, et al. (2003) BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation. Mol Cell Biol 23(16):5664-5679. PMID: 12897139
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Brown DD, Binder O, Pagratis M, Parr BA, & Conlon FL (2003) Developmental expression of the Xenopus laevis Tbx20 orthologue. Developmental Genes Evol 212(12):604-607. PMID: 12536325
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Wilson V & Conlon FL (2002) The T-box family. Genome Biol 3(6):REVIEWS3008.1-7. PMID: 12093383
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Conlon FL, Fairclough L, Price BM, Casey ES, & Smith JC (2001) Determinants of T box protein specificity. Development 128(19):3749-3758. PMID: 11585801
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Smith JC, Conlon FL, Saka Y, & Tada M (2000) Xwnt11 and the regulation of gastrulation in Xenopus. Philos Trans R Soc Lond B Biol Sci 355(1399):923-930. PMID: 11128985
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Conlon FL & Kessler DS (2000) Hopping into the new millennium. Eighth International Xenopus Conference, Estes Park, Colorado, 16-20 August 2000. Trends Genet 16(12):537-540. PMID: 11188367
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Conlon FL & Smith JC (1999) Interference with brachyury function inhibits convergent extension, causes apoptosis, and reveals separate requirements in the FGF and activin signalling pathways. Developmental Cell Biol 213(1):85-100. PMID: 10452848
Casey ES, O'Reilly MA, Conlon FL, & Smith JC (1998) The T-box transcription factor Brachyury regulates expression of eFGF through binding to a non-palindromic response element. Development 125(19):3887-3894. PMID: 9729496
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Smith JC, et al. (1997) Upstream and downstream from Brachyury, a gene required for vertebrate mesoderm formation. Cold Spring Harb Symp QuantBiol 62:337-346. PMID: 9598367
Morgan BA, et al. (1996) Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse. Proc Natl Acad Sci U S A 93(7):2801-2806. PMID: 8610121
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Conlon FL, Sedgwick SG, Weston KM, & Smith JC (1996) Inhibition of Xbra transcription activation causes defects in mesodermal patterning and reveals autoregulation of Xbra in dorsal mesoderm. Development 122(8):2427-2435. PMID: 8756288
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Conlon FL, Jones CM, & Smith JC (1996) From mouse to frogs: identification and functional analyses of genes required for induction and patterning of the mesoderm. Semin Developmental Cell Biology 7(1):95-101. ScienceDirect link
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Conlon FL, Wright CV, & Robertson EJ (1995) Effects of the TWis mutation on notochord formation and mesodermal patterning. Mech Dev 49(3):201-209. PMID: 7734393
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Conlon FL & Beddington RS (1995) Mouse gastrulation from a frog's perspective. Semin Developmental Cell Biol 6(4):249-256. ScienceDirect link
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Ruiz JC, Conlon FL, & Robertson EJ (1994) Identification of novel protein kinases expressed in the myocardium of the developing mouse heart. Mech Dev 48(3):153-164. PMID: 7893599
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Conlon FL, et al. (1994) A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. Development 120(7):1919-1928. PMID: 7924997
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Robertson EJ, Conlon FL, Barth KS, Costantini F, & Lee JJ (1992) Use of embryonic stem cells to study mutations affecting postimplantation development in the mouse. Ciba Found Symp 165:237-250; discussion 250-5. PMID: 1516471
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Conlon FL, Barth KS, & Robertson EJ (1991) A novel retrovirally induced embryonic lethal mutation in the mouse: assessment of the developmental fate of embryonic stem cells homozygous for the 413.d proviral integration. Development 111(4):969-981. PMID: 1879365